Protein IL12B

URN urn:agi-llid:3593
Name IL12B
Description interleukin 12b
Connectivity 479
Notes A direct role for IL12B in susceptibility to inflammatory bowel disease is unlikely. A direct role for IL12B in susceptibility to inflammatory bowel disease is unlikely. A key mechanism in the pathogenesis of MS is the increased expression of CD86 and CD40L and the increased production of IL12 during disease progression. ATPgammaS potentiated the IL-12p40 release induced by TNF-alpha. This potentiation was observed as long as the IL-12p40 concentration under agonist stimulation remained below a threshold value close to 10 ng/ml; inhibition was observed above this value. DISCUSSION FORUM: it is the p40 protein, either alone or paired with other polypeptides, rather than p75, that acts as an interface between the innate and adaptive immune responses. Data show that reverse signaling via 4-1BB-ligand enhanced interleukin-12beta mRNA and the secretion of IL-12 p70 in various antigen-presenting cells, including monocytes. Decreased LPS-induced IL-12(p70) and IL-10 responses were associated with the TLR4 (Asp299Gly) polymorphism and independently with asthma, especially atopic asthma in school-age children. Diesel exhaust particles seem to inhibit the production of IL-12 p40 in lipopolysaccharide activated monocytes as a model for lung macrophages. Frequencies of the various genotypes for the promoter region of the gene encoding IL-12p40 (IL12B) did not differ between psoriasis patients and controls. Heparins (unfractionated heparin, UFH and low molecular weight heparin certoparin) had no effect on the release of IL-1ra, IL-10, and IL-12p40 from neutrophils or peripheral blood mononuclear cells. Heterozygosity of a polymorphism in the 3'untranslated region of the IL12B gene was associated with a delayed disease in late-onset diabetics. IL-12 expression in PMN of SLE patients was less prominent than that of the normal controls. IL-12B 3'untranslated region has no effect or has a negligible effect on human susceptibility to tuberculosis. IL12B SNP is associated with susceptibility to atopic dermatitis and psoriasis vulgaris, presumably by affecting the Th1/Th2 balance. IL12B promoter heterozygosity contributes to asthma severity rather than susceptibility per se. Interleukin-10 induction of endothelial nitric-oxide synthase expression attenuates CD40-mediated interleukin-12 synthesis in human endothelial cells. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p40 secretion is strain and species dependent. No association of IL12B polymorphisms and self-limited HCV infection could be demonstrated. STATE OF ART REVIEW. Malignant B-cells from follicular and marginal zone lymphomas expressed IL-12 p35 and p40 transcripts, whereas only p35 mRNA was detected in mantle cell lymphoma. The IL12B 1188 (A-->C) gene polymorphism is not involved in defining the genetic basis of the susceptibility to and final outcome of peptic ulcer disease. The IL12B TaqI gene polymorphisms do not appear to be involved in susceptibility to celiac disease. The IL12B gene does not confer susceptibility to coeliac disease. The polymorphism of IL12B (1188A/C) appears to have some influence on the outcome of hepatitis c virus infection. The pro-inflammatory nature of hResistin was further evident from the ability of this protein to induce the nuclear translocation of NF-kappaB transcription factor as seen from electrophoretic mobility shift assays. The purified lipoprotein, Lpk, from Mycobacterium leprae, was found to induce production of interleukin-12 in human peripheral blood monocytes. This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. We found a strong association between the IL12B_4237 and IL12B_6402 polymorphisms and an asthma-severity phenotype in whites. Association between IL12B intron 2 polymorphism and tuberculosis and between specific IL12B haplotypes and TB found in Hong Kong Chinese population. Chronically infected patients were significantly more likely than those with resolved HCV infection to be homozygous for the IL-12B 3'-UTR A allele. Identify erythroid Kruppel-like factor as a transcription factor in macrophages able to regulate IL-12 p40 transcription depending on the cellular activation status. Inherited interleukin-12 deficiency: IL12B genotype and clinical phenotype of 13 patients from six kindreds. Interleukin 12 and interferon gamma are involved in inhibition of cytotrophoblastic cell invasion. Interleukin-12 p40 transcription and NF-kappaB activation are inhibited by nitric oxide in macrophages and dendritic cells. No evidence to support the presence of a heterozygote effect of the IL12B promoter polymorphism on the level of asthma in early childhood or adulthood. P300 is involved in the transcriptional regulation of IL-12 p40, and IL-12 p40 is one of the target genes of p300. Possible role of IL-12 and tissue antioxidants in development and progression of chronic sinusitis. Results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression. Results suggest that impaired IL-12 production in HIV-infected myeloid cells occurs, in part, via disruption of IL-12 p40 gene expression in a manner that requires cellular infection. Role of CD28 and IL-12/IL-15 signaling impairment in T cell proliferative deficiency during senescence. Study shows that PBMCs of patients with advanced gastric cancer show poor production of TNF and IL-12p40 in response to stimulation with tumor cells in vitro & this unresponsiveness is associated in some patients with diminished IRAK-1 expression in vivo. Synthesis in RAW264.7 cells is induced by IRF-8.

KEGG pathway Type I diabetes mellitus
Cytokine-cytokine receptor interaction
Jak-STAT signaling pathway
Toll-like receptor signaling pathway

Swiss-Prot Accession P29460
Q8N0X8
P43432
Q3ZAX5
Q9R278
Q62712

Unigene ID Hs.674
Mm.239707
Rn.48686

KEGG ID hsa:3593
mmu:16160
rno:64546

Swiss-Prot ID IL12B_HUMAN
IL12B_MOUSE
Q3ZAX5_MOUSE
Q9R278_RAT

Cell Localization Extracellular

GO ID 0005125
0004896
0042164
0005143
0005515
0046982
0042803
0004871
0042088
0042093
0007050
0016477
0007166
0002323
0048662
0042104
0045785
0045078
0032729
0032816
0002860
0034393
0042035
0010224
0019233
0005576
0043514
0016020
0042102
0005615
0050671
0030101
0019735
0016021
0042095
0005524
0004672
0004674
0006468

Pathway IL12R -> NF-kB/NFATC signaling
IL12R -> STAT signaling
PPARG Targets
VDR Targets

IPI ID IPI00021798
IPI00120356
IPI00207823

Ariadne Ontology Cytokine

Mouse chromosome position 11 19.0 cM

GO Molecular Function protein binding
cytokine receptor activity
interleukin-12 alpha subunit binding
protein homodimerization activity
protein heterodimerization activity
cytokine activity
interleukin-12 receptor binding
signal transducer activity

Homologene ID 1648

Hugo ID 5970

GO Cellular Component extracellular region
membrane
extracellular space
interleukin-12 complex

MGI ID 96540

Human chromosome position 5q31.1-q33.1

GO Biological Process positive regulation of cell adhesion
negative regulation of smooth muscle cell proliferation
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
positive regulation of natural killer cell activation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of smooth muscle cell apoptosis
positive regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma production
regulation of cytokine biosynthetic process
cell surface receptor linked signal transduction
natural killer cell activation during immune response
T-helper cell differentiation
cell cycle arrest
cell migration
T-helper 1 type immune response
sensory perception of pain
response to UV-B

Rat chromosome position 10q21

GenBank ID NC_000005
NM_002187
NP_002178
NT_023133
NW_001838954
NW_922784
AC_000048
AC_000137
AC011418
AF512686
AAM34792
AY008847
AAG32620
AY064126
CH471062
EAW61576
U89323
AF180563
AAD56386
AY046592
AAL05890
AY046593
AAL05891
BC067498
AAH67498
BC067499
AAH67499
BC067500
AAH67500
BC067501
AAH67501
BC067502
AAH67502
BC074723
AAH74723
M65272
AAA35695
M65290
AAA59938
P29460
Q8N0X8
NC_000077
NM_008352
NP_032378
NT_096135
NW_001030461
AC_000033
AL607030
CAM20288
AL669944
CAM22832
CH466575
EDL33847
EDL33848
D63333
S82421
AAB37383
S82422
S82424
S82425
S82426
AF128214
AAF22555
AF128215
AAF22556
AK162981
BAE37146
BC103608
AAI03609
BC103609
AAI03610
BC103610
AAI03611
BC103614
AAI03615
M86671
AAA39296
P43432
Q3ZAX5
NC_005109
NM_022611
NP_072133
NW_047334
NW_001084656
AC_000078
CH473948
EDM04146
EDM04147
AF133197
AAD24190
U16674
AAA52229
Q62712
Q9R278
AK155593
NT_086681
NW_000039

Group Cytokine
extracellular region
membrane
extracellular space
interleukin-12 complex
protein binding
cytokine receptor activity
interleukin-12 alpha subunit binding
protein homodimerization activity
protein heterodimerization activity
cytokine activity
interleukin-12 receptor binding
signal transducer activity
positive regulation of cell adhesion
negative regulation of smooth muscle cell proliferation
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
positive regulation of natural killer cell activation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of smooth muscle cell apoptosis
positive regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma production
regulation of cytokine biosynthetic process
cell surface receptor linked signal transduction
natural killer cell activation during immune response
T-helper cell differentiation
cell cycle arrest
cell migration
T-helper 1 type immune response
sensory perception of pain
response to UV-B

RGD ID 628704
61304

LocusLink ID 3593
16160
64546
116997

Alias CLMF
NKSF
CLMF2
NKSF2
IL-12B
interleukin 12B
IL12, subunit p40
OTTHUMP00000160820
interleukin 12, p40
interleukin-12 beta chain
natural killer cell stimulatory factor, 40 kD subunit
p40
Il12p40
Il-12p40
RP23-388G23.1
IL-12 p40
IL-23 subunit p40
OTTMUSP00000005619
Il12
interleukin-12 p40
Cytotoxic lymphocyte maturating factor 40 kDa subunit
cytotoxic lymphocyte maturating factor 2
cytotoxic lymphocyte maturating factor II
cytotoxic lymphocyte maturating factor 2, p40
I12B_MOUSE
Interleukin IL12 beta
Cytotoxic lymphocyte maturation factor 40 kDa subunit
cytotoxic lymphocyte maturation factor 2
cytotoxic lymphocyte maturation factor 2, p40
I12B
interkeukin-12 beta chain
Interleukin IL12AB
Interleukin-12 beta
NK cell stimulatory factor chain 2
I12B_HUMAN
IL23, subuint p
cytotoxic lymphocyte maturation factor 2, p
NK cell stimulatory factor chain II
IL23, subuint p40
natural killer cell stimulatory factor-2
cytotoxic lymphocyte maturation factor II
natural killer cell stimulatory factor II
Il12b
bronchial hyperresponsiveness 1
Bronchial hyperresponsiveness-1 (bronchial asthma)
bronchial hyperresponsiveness I
BHR I
BHR1
natural killer cell stimulatory factor
IL-12-specific subunit p40
Interleukin 12
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
CLMF P40
subunit p40
interleukin-12 p40 precursor

Organism Homo sapiens
Mus musculus
Rattus norvegicus

OMIM ID 161561
600807
209950
177900